Journal: Epigenetics & Chromatin

Article Title: Bmi1 controls auditory sensory epithelial cell proliferation through genome-wide H3K27me3 modifications

doi: 10.1186/s13072-025-00642-1

Figure Lengend Snippet: Changes in gene expression in the sensory epithelium cells of Bmi1 knockout mice. A Principal component analysis. B Volcano plot of the differentially expression genes in Bmi1 −/− mice compared to WT mice. C Heatmap of the top 25 most significantly differentially expressed genes. D Top 10 GO terms for upregulated genes and 10 GO terms for downregulated genes in Bmi1 −/− mice compared to WT mice. E – H Heatmap showing genes that were significantly upregulated in the GO terms of neurotransmitter transport, regulation of neuroscience, regulation of cell growth, and negative regulation of nervous system development in Bmi1 −/− mice compared to WT mice. I Heatmap showing genes that were significantly decreased in the GO terms of regulation of sodium ion transmembrane transport in Bmi1 −/− mice compared to WT mice. J KEGG pathway analysis of differential expressed genes between Bmi1 −/− and WT mice. K A representative gene interaction network diagram showed an increased expression of Cdkn2c in Bmi1 −/− mice compared to WT mice, with Cdkn2c interacting with other proteins. In the Figure, "HO" represented Bmi1 −/− group

Article Snippet: Transfections were performed using 200 nM siRNA targeting Cdkn2c or a non-targeting control siRNA, both obtained from Sangon Biotech (Supplementary Table 1).

Techniques: Gene Expression, Knock-Out, Expressing

Journal: Epigenetics & Chromatin

Article Title: Bmi1 controls auditory sensory epithelial cell proliferation through genome-wide H3K27me3 modifications

doi: 10.1186/s13072-025-00642-1

Figure Lengend Snippet: H3K27me3 involvement in the regulation of gene transcription in the inner ear. A H3K27me3 was ubiquitously expressed in the auditory sensory epithelium of P2 neonatal mice inner ear. Hair cells are labeled with Myosin VIIa in gray, H3K27me3 is labeled in green, and support cells are labeled with Sox2 in red. B Genomic distribution of H3K27me3 signals in the auditory sensory epithelium of P2 neonatal mice inner ear. C Representation of H3K27me3-rich genes ranked by Mean Reciprocal Rank. Genes highlighted in red ( Pax2 , Cdkn2a , Cdkn2b , and Cdkn2c ) were specifically labeled because of their well-established functions in regulating cell proliferation and the cell cycle. D GO analysis of genes with H3K27me3 signals. E KEGG pathway analysis of genes with H3K27me3 signals. Screenshot showing the H3K27me3 signals of the Pax2 ( F ), Cdkn2a , Cdkn2b ( G ) and Cdkn2c ( H ) gene sequences and nearby regions

Article Snippet: Transfections were performed using 200 nM siRNA targeting Cdkn2c or a non-targeting control siRNA, both obtained from Sangon Biotech (Supplementary Table 1).

Techniques: Labeling

Journal: Epigenetics & Chromatin

Article Title: Bmi1 controls auditory sensory epithelial cell proliferation through genome-wide H3K27me3 modifications

doi: 10.1186/s13072-025-00642-1

Figure Lengend Snippet: Integrated analysis of RNA-seq, ChIP-seq and ATAC-seq. A Gene Set Enrichment Analysis of genes with decreased H3K27me3 marks in Bmi1 −/− group. B Volcano plots indicating the overlap of gene identities from differential genes and peak annotations from RNA-seq and ATAC-seq in the Bmi1 −/− group compared to the WT group, respectively. Gene and peak annotations were categorized as either increased or decreased based on the direction of expression and accessibility change. C Volcano plots indicating the overlap of gene identities from differential genes and peak annotations from RNA-seq and H3K27me3-ChIP-seq in the Bmi1 −/− group compared to the WT group, respectively. Gene and peak annotations were categorized as either increased or decreased based on the direction of expression and H3K27me3 signals. D Analysis of the overlap between upregulated genes, loss of H3K27me3, and increased chromatin accessibility in Bmi1 −/− group. E Network analysis of the interaction between genes with increased expression in RNA-seq and genes with decreased H3K27me3 marks in ChIP-seq in the Bmi1 −/− group compared to the WT group. F Screenshot showing the RNA expression peak, chromatin accessibility, and H3K27me3 signals of the Cdkn2c gene in the genome of WT and Bmi1 −/− groups. G Top six motifs from ATAC-seq data in the WT group and the Bmi1 −/− group, respectively

Article Snippet: Transfections were performed using 200 nM siRNA targeting Cdkn2c or a non-targeting control siRNA, both obtained from Sangon Biotech (Supplementary Table 1).

Techniques: RNA Sequencing, ChIP-sequencing, Expressing, RNA Expression

Journal: Epigenetics & Chromatin

Article Title: Bmi1 controls auditory sensory epithelial cell proliferation through genome-wide H3K27me3 modifications

doi: 10.1186/s13072-025-00642-1

Figure Lengend Snippet: Inhibition of Cdkn2c promoted the proliferating ability in epithelial cells of Bmi1 −/− OC. A RT-qPCR showed that the mRNA expression levels of Bmi1 was significantly decreased and the mRNA expression levels of Cdkn2c was significantly increased in Bmi1 −/− group when compared with WT group. B Western blots showed that the protein expression level of p18 INK4c was significantly increased in Bmi1 −/− group when compared with WT group. C Relative quantification of p18 INK4c density in WT and Bmi1 −/− group based on Western Blots. D , E Immunofluorescence staining showing increased p18 INK4c expression in supporting cells in Bmi1 −/− group. Apex: apical turn; Mid: middle turn; Base: basal turn. F Relative immunofluorescence quantification of p18 INK4c in WT and Bmi1 −/− group. G Relative expression of Cdkn2c mRNA after siRNA treatment. siRNA2 significantly reduced the p18 INK4c mRNA expression level in Bmi1 −/− cochlear explants. H The images showed typical representations of spheres across WT, Bmi1 −/− and siRNA2 treated Bmi1 −/− groups. I The quantification of sphere areas revealed that the spheres in the Bmi1 −/− group were significantly smaller compared to those in the WT group. However, the introduction of siRNA2 notably increased the sphere area in the Bmi1 −/− group. J Immunofluorescence staining showing the Sox2 + cells, EdU + cells and Myo7a + cells of spheres in WT, Bmi1 −/− and siRNA treated Bmi1 −/− group. K The quantification of Sox2 + and EdU + cells within the spheres demonstrated that the numbers of both Sox2 + and EdU + cells in the Bmi1 −/− group were significantly lower compared to the WT group. However, treatment with siRNA2 notably increased the numbers of Sox2 + and EdU + cells in the Bmi1 −/− group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data shown as mean ± SEM. "n" represents the number of biological replicates analyzed. Scale bars were shown in sub-figures

Article Snippet: Transfections were performed using 200 nM siRNA targeting Cdkn2c or a non-targeting control siRNA, both obtained from Sangon Biotech (Supplementary Table 1).

Techniques: Inhibition, Quantitative RT-PCR, Expressing, Western Blot, Quantitative Proteomics, Immunofluorescence, Staining